Next generation sequencing services for DECIDER

The following services will be purchased (estimated number of analysis per year in parentheses). Quality control measurement, library preparation and high-throughput sequencing for Whole Genome Sequencing (WGS, ~250 samples), Whole Genome Bisulfite Sequencing (WGBS, ~100 samples), and RNA sequencing (RNA-seq, ~250 samples) services. DNA extraction, quality control measurement, library preparation and high- throughput sequencing of ctDNA samples for whole-genome shallow sequencing (sWGS, ~100 samples), whole-exome sequencing (WES, ~25 samples) and targeted sequencing including at least exonic regions of 200 cancer-specific genes (~5 samples). The study is part of an ongoing EU project led by Professor Sampsa Hautaniemi at the University of Helsinki. The project builds on existing data received from previous sequencing projects by EU project HERCULES which was produced according to the procedures described in the invitation to tender section VI.4) Other information: "Content of the services", and thus the sequencing data purchased from the service provider should be compatible with these data types (not applicable to ctDNA-specific sequencing). An agreement for 2 years will be signed of the services. One or many services will be ordered in smaller batches. For each order a separate sub-agreement needs to be prepared and signed to define requested services, number of samples, up-to-date prices and deadline for data delivery.