High resolution LC-MS-MS for metabolomics and proteomics

The LC-high resolution (HR) MS/MS -system will be used for metabolomics analysis as well as for analysis of proteins and peptides. The instrument is suitable for non-targeted profiling analysis and reliable identification of metabolites, and proteins and software is suitable for data acquisition and exploration and ease of data export.

The new equipment is replacing our current system: UPLC-DAD-/nano-UPLC- ESI-QTOF MS/MS. The LC-DAD-HR MS/MS system is used for metabolomics analysis as well as for analysis of proteins and peptides. For proteomics, connecting the existing Waters Acquity nano-UPLC to the HR MS/MS can be included to the purchased system. The instrument is suitable for non-targeted profiling analysis and reliable identification of specialized metabolites and software is suitable for data acquisition and exploration and ease of data export. The instruments and software were evaluated for their ability to separate, identify, and semi-quantitate metabolites with high confidence in metabolomics analysis. The instruments and software were evaluated for the ability to separate and identify peptides and proteins with high confidence. Straightforward MS/MS strategy and easy combination of negative and positive ionization data for identification was appreciated. Good sensitivity, wide linear range of quantitation, and signal intensity and retention time reproducibility was necessary. For analysis of proteins and peptides, instrument is operating at nano-flow or similar capabilities. Analysis software can use for non-label quantification and provide list of detected proteins. Data export in various formats for possible additional analytical needs was desirable.