Exome sequencing reagents - NGS library adapters and hybridization probes

This public tender process was for NGS Library Adapters (also "Adapters") and Hybridization Probes (also "Probes") used in the diagnostic whole exome sequencing (WES) NGS pipelines of HUSLAB, Laboratory of Genetics. The pipeline is used for preparing high quality sample libraries for Illumina NovaSeq 6000 sequencer. The HUSLAB WES workflow is based on a target enrichment step using hybridization probes to enrich DNA fragments originating from the genomic Regions of Interest ("RoI"). HUSLAB WES is used for high sensitivity variant detection of both germline and somatic DNA variations. The Contracting Entity estimates that annual orders will be made for 2000 samples (estimation for 2022 and HUS estimates, that the number of samples is rising) divided in three to four (3-4) different separate orders. The tender is devided into two lots: 1) NGS Library Adapters 2) Hybridization Probes The procurement entity selects one supplier for both lots so that the lot may have either the same or a different supplier. INTENDED USE The Adapters and the Probes offered will be Components of the HUSLAB WES process. HUSLAB will select the Components and combine them in order to create the final HUSLAB WES process used for generation of high quality and high sensitivity customized WES sample libraries, fulfilling the Special Requirements for High Sensitivity Variant Detection. The WES pipelines using the Adapters and the Probes are used for production of high quality WES sample libraries from DNA isolated from: - whole blood - freshly isolated or frozen tissue samples or cells - FFPE samples (optionally omitting DNA fragmentation) - cell-free DNA (omitting input DNA fragmentation) The NGS pipelines using the Components allow for HUSLAB to build NGS pipelines fulfilling the accreditation requirements of SFS-EN ISO 15189:2012.