PacBio Revio (Pacific Biosciences)

Justification for the direct purchase. The justification must be based on one of the preconditions mentioned in the procurement act (1397/2016).( 40§, 41§, 110§, 119§). 40§ 2) only a certain supplier can implement the procurement for a technical reason, or for a reason related to protecting an exclusive right; it shall be a further condition that there are no reasonable alternatives or substitute solutions, and that the absence of competition is not due to an artificial narrowing of the terms and conditions of the procurement. The DNA Sequencing and Genomics laboratory is planning to replace its long read single molecule sequencing instrument. Requirements for the new instrument are: • Instrument that can sequence long DNA sequences with read lengths even more that 300 000 bp. • Long insert sizes (up-to 20-40 kbp) must be allowed to be used. • Same molecule can be sequenced several times increasing the quality and accuracy of the obtained data (at least 90% of bases >Q30). • It must have sequencing accuracy of very high quality (average Q40 and above). • DNA modifications can also be deduced from the obtained data • Deep consensus analysis need to be run on board the machine not dependable of outside clusters for computing. The PacBio Revio is the only instrument in the market that has all the features that are required for the needed sequencing service.

The DNA Sequencing and Genomics laboratory is planning to replace its long read single molecule sequencing instrument. Requirements for the new instrument are: • Instrument that can sequence long DNA sequences with read lengths even more that 300 000 bp. • Long insert sizes (up-to 20-40 kbp) must be allowed to be used. • Same molecule can be sequenced several times increasing the quality and accuracy of the obtained data (at least 90% of bases >Q30). • It must have sequencing accuracy of very high quality (average Q40 and above). • DNA modifications can also be deduced from the obtained data • Deep consensus analysis need to be run on board the machine not dependable of outside clusters for Page 2 2 computing. The PacBio Revio is the only instrument in the market that has all the features that are required for the needed sequencing service.