FACSDiscover A8 Cell analyzer with CellView, 5 lasers, 78 detectors

FACSDiscover A8 Cell analyzer with CellView, 5 lasers, 78 detectors from Becton Dickinson Finland Oy

The required performance and interoperability can be fulfilled only by one supplier, Water Biosciences, and no reasonable alternative or substitute exists. The device must meet the following mandatory requirements, which are essential for our research workflows and for interoperability with our existing infrastructure: • a necessary upgrade to our flow cytometry capabilities, introducing proprietary imaging technology and a seamless integration with our existing BD FACSDiscover S8 Cell Sorter allowing transfer of panels between the two instruments. • high-throughput imaging integrated with full-spectrum flow cytometry, operating 10-100 times faster than camera-based systems. The BD FACSDiscover A8 shares the same optical configuration, calibration standards, and FACSChorus software platform as the FACSDiscover S8 cell sorter. This standardization allows for seamless transfer of validated panels from the A8 to the S8 and vice versa without re-optimizing, a unique advantage among analyzer-sorter combinations. Researchers can develop panels, set up compensation, and screen samples on the A8, then confidently sort on the S8, ensuring data reproducibility. BD's "perform as one" calibration philosophy facilitates experiment transferability within our facility and across all FACSDiscover systems globally, enhancing multi-site collaborations and reproducibility. The superior imaging capabilities of the A8, and the identical optical architecture of BD FACSDiscover A8, is essential for analyses process in our infrastructure. The CellView™ Imaging parameters make it possible to use completely new parameters for identifying populations of interest. In addition to the fluorescent parameters normally measured with a flow cytometer this instrument allows new spatial dimensions with parameters such as diffusivity, max intensity, radial moment, and 12 other automatically generated parameters. These parameters are crucial for several types of experiments in the infrastructure. We already have experiments from several research groups waiting for this instrument to the research infrastructure since they need parameters that cannot be measured with any other instrument on the market. These experiments include e.g. • Location and translocation assays. Cells analyzed should have the desired protein located in the cytoplasm or the nucleus. With CellView cells with the correct expression pattern can be selected for analysis. Without images, the location is not known. • Identification of real cell-cell interactions with high throughput speed. This is crucial for ongoing immuno-oncology studies of interaction of T and NK-cells with cancer tumor cells. In addition we get frequent requests for different cell-immune cell interaction analysis. Without images, real cellcell interactions are not distinguished from doublets. • Studies and identification of chromatophores morphological properties. This experimental setup relies totally on the image parameters. • Image verification of the rare event populations. This is invaluable for several researchers analysing very rare cell populations. They get an immediate verification based on the image whether the event is real.