Sequencing instrument

Tampere University (contracting authority) is publishing direct award notice for 'Sequencing instrument'.

BACKGROUND To increase sequencing capacity in-house, Tampere Genomics Facility (https://www.tuni.fi/en/research/tampere-genomics-facility) is in the process of expanding its laboratory infrastructure that is currently lacking adequate capacity. With the mid output sequencing instrument, it would be possible to conduct sequencing projects that now need to be outsourced. Such projects include, for instance, mRNA-sequencing, human genome sequencing and single-cell sequencing. One of the main aims is to elevate the level of science with faster turnaround time as having adequate sequencing capacity in-house would allow testing of new ideas in few days whereas the time frame may be months with outsourced sequencing service. NEED The instrument should have the following specifications: • The range of outputs are flexible with low, medium and high throughput kits. • Sequencing cycle outputs are flexible including 150, 300 and 600 cycle kits. • Long size inserts (up to 1.5 kbp) must be allowed to be used. • Total output range should be ~50–600 Gb. • Instrument must have two independent flow cells to allow its use in parallel or independently like two sequencers in one. • Linear libraries must be able to be loaded to the flow cell and the library circulation and RCA be made onboard. • It must have sequencing accuracy of very high quality (average Q30 >90%). • Instrument must use rolling circle amplification (RCA) that minimizes common amplification errors: no PCR error propagation, negligible index hopping, low duplicate rates, low AT/GC bias and no optical duplicates. • Instrument must be field upgradable to allow multiomic profiling (such as simultaneous detection of RNA, proteins, and cell morphology within the same biological sample). MARKET ANALYSIS AND CONCLUSION FOR DIRECT AWARD Meeting all the specified requisites and the ability to provide all required properties is mandatory of been selected in this procurement process. The AVITI System from Element Biosciences is the only instrument in the market that has all the features that are required for the sequencing instrument needed.