The high-resolution, accurate mass Orbitrap Astral Zoom Mass Spectrometer with FAIMS Pro Duo

University is planning to buy The high-resolution, accurate mass Orbitrap Astral Zoom Mass Spectrometer with FAIMS Pro Duo for Turku Proteomics facility, Turku Bioscience Centre. Turku Bioscience Centre is an advanced core facility and research centre hosted jointly by University of Turku and Åbo Akademi University.

The high-resolution, accurate mass Orbitrap Astral Zoom Mass Spectrometer with FAIMS Pro Duo, provided by Thermo Fisher Scientific, is the only instrument in the market that meet all the specific and necessary technical requirements to fulfil the uncompromising needs of the acquiring department as follows: • Our methods have been developed for Orbitrap Astral, Orbitrap Exploris 480 and Orbitrap Fusion Lumos Tribrid mass spectrometers. These methods can be transferred more easily onto another Orbitrap Astral -type mass spectrometer to ensure continuity. • New instrument needs to be compatible with existing Nanospray Flex Ion Source, FAIMS Pro Duo interface, control software, and UHPLC systems in Turku Proteomics facility. • Our research consists of multiple workflows that require the use of TMTpro 35-plex reagents for maximum throughput, and therefore the new instrument needs to have mass analysers capable to achieve high mass resolution (100000 at 138m/z), quantitative performance, and dynamic range (>5000:1 within one spectrum). • The instrument must have two high-resolution, accurate mass analysers that operate in parallel, one of which must be able to deliver MS/MS spectra at a rate of 270Hz. • New instrument needs to provide a sensitivity increase over the existing Orbitrap Astral, as defined by protein group identifications achievable on a single-cell level input (250pg) of HeLa digest utilising the FAIMS Pro Duo interface for improved signal: noise. • The instrument must be capable of performing narrow window (2Th) DIA to increase selectivity and sensitivity for applications such as plasma proteomics. • The instrument must support internal standard-triggered targeted protein quantitation workflows with both mass analysers • The instrument must support Hybrid DIA method that enables acquisition of targeted and untargeted data in a single injection • The older instrument is used as a substantial part of payment as the seller agrees to exchange an Orbitrap Astral installed in 2025 for a new upgraded one.