Genotyping service procurement of Atlantic salmon and herring, perch, sprat and brown trout for Natural Resources Institute Finland

Natural Resources Institute Finland (hereafter referred to as "Luke") requests your tender for genotyping of fish with this Call for Tenders and its annexes. The Call for Tenders and the procurement is divided into two (2) lots. 1. Genotyping of Atlantic salmon 2. Genotyping of herring, perch, sprat and brown trout Tenderer can leave a tender for one or both of the lots. One supplier is chosen to each lot. The subject of the procurement is described in more detail in this call of tenders and other procurement documents.

The subject of the procurement is genotyping service of fish. The subject of the procurement is described in more detail in call of tenders and other procurement documents. Luke is looking for a service provider for genotyping service of Atlantic salmon (Salmo trutta), herring (Clupea harengus), perch (Perca fluviatilis), sprat (Sprattus sprattus) and brown trout (Salmo trutta) individuals with a minimum of 67,000 SNP markers for salmon and 1,000 SNP for other species. The genotype data of individuals will be used to identify population structures of different fish species and combined with other, e.g. phenotypic data from the genotyped individuals. To make powerful genomic analysis across generations and data sets, the genotype data produced by the service provider will be combined by Luke with the Luke's existing genotype data sets generated previously by Atlantic salmon SNP array, with total SNP density of approximately 70K SNPs, and for brown trout with by a multispecies SNP array with a density of approximately 4,000 SNPs. The samples sent to the service provider can be potentially used again by Luke in some future laboratory analysis, and hence they remain valuable for Luke even after the service providers have completed their analysis. Lot 1 Genotyping of Atlantic salmon -The SNP panel to be used by the service provider should have a minimum of 67,000 SNP markers to ensure a reasonable coverage of the Atlantic Salmon genome. -The 67,000 SNPs need to be suitable for genotyping of Atlantic salmon stocks of the Baltic Sea, including distinguishing the hatchery stocks from natural populations. The data from the 67,000 SNP markers need to be compatible with the Atlantic salmon SNP array data already existing at Luke. -Luke will send tissue samples (fins or scales) of individuals or DNA to the service provider either in 1.5ml centrifuge tubes or on well plates. The samples will be sent in batches of around 384 fish. Luke covers the shipment costs. Lot 2 Genotyping of herring, perch, sprat and brown trout - The SNP panel to be used by the service provider should have a minimum of 1,000 SNP markers per species to ensure a reasonable coverage of the genome. - The 1,000 SNP markers need to include the SNP markers in the DNA TRACEBACK® Fisheries SNP genotyping array FSHSTK1D. -Luke will send tissue samples (fins or scales) of individuals or DNA to the service provider either in 1.5ml centrifuge tubes or on well plates. The samples will be sent in batches of around 384 fish. Luke covers the shipment costs. The service provider will in both lots: - Extract DNA from the individual fin clips or fish scales - Quality control analyses - Provide genotyping data in an electronic format - Return of the remaining tissue or DNA of each sample to Luke upon request and cover the shipment costs of returning the samples Luke remains the owner of the data, and the service provider does not own the data.

The call for tenders has been amended to allow the submission of reference information as an attachment